王雪颖 副研究员
个人简历
2006-2010 大连大学 学士学位
2010-2018 大连化学物理研究所 博士学位
2018-2021 大连化学物理研究所1816组 助理研究员
2021-2023 大连化学物理研究所1816组 副研究员
2023-至今 大连化学物理研究所1823组 副研究员
研究方向：酵母细胞工厂的构建以及甲醇生物转化研究
电话：0411-84379066
代表文章
1. Wu Y.J., Wang X.Y., Yang K.G., Sui Z.G.*, Yan H.M.*, Zhang L.H., Zhang Y.K., Automated strain-to-peptide conversion: a high-throughput proteome analysis platform empowering rational design of microbial cell factories, Bioresour. Technol., 2026, 444, 134010.
2. Wu X.H., Hu Y.H., Huang Y.Z., Zhang L.Y., Xue Y., Cao H.L., Wang X.Y.*, Zhou Y.J., Expanding the Cofactor Range of Aldehyde Reductase YqhD by Mutating Key Amino Acids, Biotechnol Lett, 2026, doi.org/10.1007/s10529-026-03747-z.
3. Wang X.Y.*, Liu Y.Y., Hu Y.H., Huang Y.Z., Zhang L.Y., Xue H.Z., Zhou Y.J., Zhao Z.K.*, Engineering nicotinamide adenine dinucleotide oxidase for regeneration of oxidized non-natural cofactor, ChemBioChem, 2025, 26, e202500254.
4. Hu Y.H., Wang X.Y., Huang Y.Z., Guo X.J., Xue H.Z., Martin P., Bernd N., Zhao Z.K.*, Enzymatic synthesis and characterization of nicotinamide riboside pseudouridine diphosphate, Tetrahedron Lett., 2025, 155, 155432.
5. 王雪颖，周雍进，赵宗保*，非天然氧化还原辅酶赋能生物制造，化工学报，2024，75（11），4037-4047.
6. Guo X.J., Wang X.Y., Hu Y.H., Zhang L.Y., Zhao Z.K.*, Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide, Sci. Rep., 2024, 14, 28701.
7. Wang J.T., Wan L., Guo X.J., Wang X.Y.*, Zhao Z.K., Competitive inhibition of a non-natural cofactor dependent formaldehyde dehydrogenase by imidazole, Biotechnol. Lett., 2023, 45, 679–687.
8. 付凯璇，王雪颖*，黄彦喆，薛海曌，胡英菡，赵宗保，基于理性设计提高1,3-丙二醇氧化还原酶的稳定性和活性，食品与发酵工业，2023，49（16），18–25.
9. Li Q., Guo X.J., Wang X.Y., Wang J.T., Wan L., Xue H.Z., Zhao Z.K*., Creation of cytochrome P450 catalysis depending on a non-natural cofactor for fatty acid hydroxylation, J. Energy Chem., 2023, 79, 31–36.
10. Wan L., Wang X.Y.*, Hu Y.H., Li Q., Zhao Z.K., Gram-scale biocatalytic preparation of the non-natural cofactor nicotinamide cytosine dinucleotide, Tetrahedron Lett., 2022, 88, 153368.
11. Wang X.Y., Feng Y.B., Guo X.J., Wang Q., Ning S.Y., Li Q., Wang J.T., Wang L., Zhao Z.K*, Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide, Nat. Commun. 2021, 12(1), 2116.
12. Guo X.J., Liu Y.X., Wang Q., Wang X.Y., Li Q., Liu W.J., Zhao Z.K*, Non-natural cofactor and formate-driven reductive carboxylation of pyruvate, Angew. Chem. Int. Ed., 2020, 59(8), 3143–3146.
13. Guo X.J., Wang X.Y., Liu Y.X., Li Q., Wang J.T., Liu W.J.*, Zhao Z.K*, Structure-guided design of formate dehydrogenase for regeneration of a non-natural redox cofactor, Chem. Eur. J., 2020, 26(70), 16611–16615.
14. Liu Y.X., Feng Y.B., Wang L., Guo X.J., Liu W.J., Li Q., Wang X.Y., Xue S.*, Zhao Z.K*., Structural insights into phosphite dehydrogenase variants favoring a non-natural redox cofactor, ACS Catal., 2019, 9, 1883–1887.
15. Wang X.Y., Wang L., Lin X.P., Yang X.B., Liu W.J., Zhao Z.K*, Visualizing soluble protein mutants by using monomeric red fluorescent protein as a reporter for directed evolution, Appl Biochem Biotechnol., 2018, 185(1), 81-90.
16. Wang X.Y., Zhou Y.J., Wang L., Liu W.J., Peng C., Zhao Z.K*, Engineering Escherichia coli nicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis, Appl Environ Microbiol. 2017, 81(13), e00692-17.
