Recently, we developed a two-end selection marker (Two-ESM) method for site-directed mutation of essential genes in Saccharomyces cerevisiae (Appl. Microbiol. Biotechnol., 2020 http://link.springer.com/article/10.1007/s00253-020-10405-5), which will facilitate the functional genomic analysis and metabolic flux regulation. (Text and Image by Shan Yang)

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