Recently, we characterized methanol metabolism-related promoters inOgataea polymorpha，and realizing regulation of fatty alcohol synthesis, which was published on Applied Microbiology and Biotechnology (Applied Microbiology and Biotechnology, 2021, 105, 8761-8769).
Promoters play an important role in regulating gene expression, and construction of microbial cell factories requires multiple promoters for balancing the metabolic pathways. However, there are only a limited number of characterized promoters for gene expression in O. polymorpha, which hampers the extensive harnessing of this important yeast toward a cell factory. We here characterized the promoters of methanol utilization pathway, precursor supply pathway and reactive oxygen species (ROS) defense system, by using a green fluorescence protein variant (GFPUV) as a quantification signal. Finally, the characterized promoters were used for tuning a fatty alcohol biosynthetic pathway in O. polymorpha and realized fatty alcohol production from methanol. This promoter box should be helpful for gene expression and pathway optimization in the methylotrophic yeast O. polymorpha. (Text and image by Xiaoxin Zhai)