课题组成员

栾宏伟 副研究员

个人简历

1995-1999 辽宁大学 学士学位

2002-2008 大连化物所 博士学位

2008-2012 大连化物所生物技术部 助理研究员

2012-2017 大连化物所生物技术部1806组 副研究员

2017至今 大连化物所生物技术部1823组 副研究员

研究方向:食用菌及功能食品的开发

电话:0411-88133490

电子邮箱:lhw@dicp.ac.cn

代表文章

1. Dou T.Y., Luan H.W., Ge G.B., Dong M.M., Zou H.F., He Y.Q., Cui P., Wang J.Y., Hao D.C., Yang S.L., Yang L.*, Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel, Sci Rep., 2015, 5, 13768.

2. Luan H.W., Dou T.Y., Liu X.B., Li S.Y., Du X.F., Yang L.*, Enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from Cellulosimicrobium cellulans, Biotechnol Lett., 2015, 37, 1905-1910.

3. Zhou K., Luan H.W., Hu Y., Ge G.B., Liu X.B., Ma X.C., Hou J., Wang X.L., Yang L.*,  Isolation and characterization of a novel α-glucosidase with transglycosylation activity from Arthrobacter sp. DL001, J. Mol. Cataly. B-Enzym., 2012, 80, 48-57.

4. Ge G.B., Liang S.C., Liu X.B., Hu Y., Mao Y.X., Zhang Y.Y., Luan H.W., Qiu M.H., Yang L.*, Rapid Qualitative and Quantitative Determination of Seven Valuable Taxanes from Various Taxus Species by UFLC-ESI-MS and UFLC-DAD, Planta Med., 2010, 76(15), 1773-1777.

5. Dong P.P., Ge G.B., Zhang Y.Y., Ai C.Z., Li G.H., Zhu L.L., Luan H.W., Liu X.B., Yang L.*, Quantitative structure-retention relationship studies for taxanes including epimers and isomeric metabolites in ultra fast liquid chromatography, J. Chromatogr. A., 2009, 1216, 7055-7062.

6. Hu Y., Luan H.W., Liu H.X., Ge G.B., Zhou K., Liu Y., Yang L.*, The acceptor specificity and transfer efficiency of a β-glycosidase from the China white jade snail, Biosci. Biotech. Bioch., 2009, 73(3), 671-676.

7. Hu Y., Luan H.W., Ge G.B., Liu H.X., Zhou K., Liu Y., Yang L.*, Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China White Jade Snail, J. Biotechnol., 2009, 139, 229-335.

8. Ge G.B., Luan H.W., Zhang Y.Y., He Y.Q., Liu X.B., Yang L., Wang Z.T., Yang L.*, Profiling of yew hair roots from various species using ultra-performance liquid chromatography-electrospray ionization mass spectrometry, Rapid Commun. Mass Sp., 2008, 22(15), 2315-2323.

9.Hu Y., Luan H.W., Zhou K., Ge G.B., Yang S.L., Yang L.*, Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity, Enzyme Microb. Tech., 2008, 43 (1), 35-42.

10. Ge G.B., Zhang Y.Y., Hao D.C., Hu Y., Luan H.W., Liu X.B., He Y.Q., Wang Z.T., Yang L.*, Chemotaxonomic Study of Medicinal Taxus Species with Fingerprint and Multivariate Analysis, Planta Med., 2008, 74, 773-779.

11. Hu Y., Luan H.W., Hao D.C., Xiao H.B., Yang S.L., Yang L.*, Purification and characterization of a novel Ginsenoside-hydrolyzing β-D-glucosidase from the China white jade snail (Achatina fulica), Enzyme Microb. Tech., 2007, 40, 1358-1366.

12. Luan H.W., Liu X., Qi X.H., Hu Y., Hao D.C., Cui Y., Yang L.*, Purification and Characterization of a Novel Stable Ginsenoside Rb1-Hydrolyzing β-D-glucosidase from China White Jade Snail, Process Biochem., 2006, 41, 1974-1980.

13. 栾宏伟,胡莹,刘兴宝,郝大程,杨凌, 一种来源于Leifsonia shinshuensis DICP 16菌体的β-D-木糖苷酶的分离纯化及其性质, 生物工程学报2008, 24(5), 867-873.

14. 刘兴宝,栾宏伟,葛广波,侯琨,杜逊甫,杨凌, 工业色谱法分离制备7-木糖基-10-去乙酰紫杉醇酶解产物10-去乙酰紫杉醇, 色谱2012, 30(2), 165-169.

15. 刘兴宝,栾宏伟,葛广波,闫黎,杨凌, 大孔吸附树脂提取7-木糖-10-去乙酰紫杉醇酶解产物10-去乙酰紫杉醇,天然产物研究与开发2009, 21(3), 496-501.

16. 李洪刚,杨义,何克江,栾宏伟,杨凌, 分组和单体人参皂苷的批量制备,首都医药2004, 11(14), 52-53.

17. 李洪刚,何克江,韩蔚,栾宏伟,杨凌, 树脂联用纯化人参总皂苷,首都医药2004, 11(12), 48-51.

18. 何克江,韩 蔚,栾宏伟,杨凌, 纯化和分光光度法检测联用的人参总皂苷的简易测定方法,药物分析杂志2003, 23(4), 284-288.

专利

1. 栾宏伟, 张辉, 一种从红豆杉属植物中提取紫杉醇及其衍生物的方法,专利号:201310067916.4

2. 杨凌,何克江,杨义,栾宏伟,  用酶水解人参皂甙制备20-β-D-吡喃葡萄糖基原人参二醇,专利号:ZL01133410X3. 杨凌,周坤,栾宏伟, 一种用于烷基糖苷合成的微生物及其应用,专利号:201110030590.9

4. 杨凌,侯琨,栾宏伟, 一种生产7-木糖-10-去乙酰紫杉醇的方法及其专用培养基,专利号:201210566819.5

5. 杨凌,窦同意,栾宏伟,刘兴宝,侯琨, 一种纤维化纤维微细菌菌株及其应用,申请号:201310245307.3

7. 杨凌,窦同意,栾宏伟,刘兴宝,李世阳, 一种木糖苷酶Xyl_S及其编码基因与应用,申请号:201310251300.2